BLAST+ Command Line Applications The NCBI Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families. BLAST+ is a new suite of BLAST tools that utilizes the NCBI C++ Toolkit. The BLAST+ applications have a number of performance and feature improvements over the legacy BLAST applications (ncbi-blast). For details and citation, please see the BLAST+ user manual from the program's web site and the article in BMC Bioinformatics. This just repackages the binaries provided from upstream.